| File | Action |
|---|---|
| Swimming Through Science: Unveiling the Secrets of Endocrine Disruptors with Zebrafish Models | Download |
- 919088951040 call us
- chronicleofaquaticscience@gmail.com Mail us
Swimming Through Science: Unveiling the Secrets of Endocrine Disruptors with Zebrafish Models
Review
Manuscript ID: CoAS_SP1_11
Swimming Through Science: Unveiling the Secrets of Endocrine Disruptors with Zebrafish Models
Tejal Bhapkar, Swati Umap*, A. P. Somkuwar, Alka Sawarkar, Gauri Deshmukh and M. M. Purankar
Abstract
The utilization of zebrafish (Danio rerio) as a model organism in studying endocrine disruptor chemicals (EDCs) has emerged as a powerful and versatile approach in toxicology research. This review highlights the numerous advantages that make zebrafish an ideal model for assessing the impact of EDCs on endocrine systems. Zebrafish, with their high fecundity, transparent embryos and rapid development, offer a cost-effective and time-efficient platform for observing the effects of EDC exposure on various endocrine-related pathways. Furthermore, the zebrafish’s amenability to genetic manipulation has revolutionized the study of EDCs, allowing for precise control and modification of specific genes associated with endocrine function. Genetic application, such as transgenesis and CRISPR/Cas9 technology, enable researchers to create targeted mutants, providing valuable insights into the molecular mechanisms underlying endocrine disruption. This review underscores the pivotal role of zebrafish in advancing our understanding of EDCs, emphasizing the synergy between its inherent advantages and genetic tools, thus positioning it as a premier model for unraveling the complexities of endocrine disruption and facilitating the development of targeted interventions.
Keywords
Zebrafish, Endocrine Disruptor Chemicals, Endocrine Disruption, Toxicology model, EDC effects
References
Antczak, P., & Meister, A. (2017). A versatile new tool to quantify ciliate ingestion by meiofauna using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Molecular Ecology Resources, 17(6), 1293-1305.
AnvariFar, H., Ahmadi, F., & Adel, M. (2020). Long-term exposure to environmentally relevant concentrations of estrogen induces metamorphosis and alters sex steroid levels in zebrafish (Danio rerio). Environmental Science and Pollution Research, 27(21), 26313-26323.
Bateson, P., Barker, D., Clutton-Brock, T., Deb, D., D'Udine, B., Foley, R. A., & Sultan, S. E. (2004).
Developmental plasticity and human health. Nature, 430(6998), 419-421.
Brion F, Tyler CR, Palazzi X, Laillet B, Porcher JM, Garric J, Flammarion P. (2004) Impacts of 17β-estradiol, including environmentally relevant concentrations, on reproduction after exposure during embryo- larval-, juvenile- and adult-life stages in zebrafish (Danio rerio). Aquat. Toxicol. 68(3), 193–217
Browne, P., Noyes, P. D., Casey, W. M., & Dix, D. J. (2017). Application of adverse outcome pathways to US
EPA’s endocrine disruptor screening program. Environmental health perspectives, 125(9), 096001.
Chakraborty, C., Hsu, C. H., Wen, Z. H., & Lin, C. S. (2009). Aggregation of Zebrafish Embryos for High- throughput Drug Toxicity Screening. Journal of Visualized Experiments, (25), e1422
Chen, Q. L., Yu, T., Zhou, J., Huang, H. W., & Wang, P. (2017). Proteomic and metabolomic responses of different zebrafish tissues to BDE-47 and TBBPA exposure. Science of the Total Environment, 574, 1147-1155.
AnvariFar, H., Ahmadi, F., & Adel, M. (2020). Long-term exposure to environmentally relevant concentrations of estrogen induces metamorphosis and alters sex steroid levels in zebrafish (Danio rerio). Environmental Science and Pollution Research, 27(21), 26313-26323.
Bateson, P., Barker, D., Clutton-Brock, T., Deb, D., D'Udine, B., Foley, R. A., & Sultan, S. E. (2004).
Developmental plasticity and human health. Nature, 430(6998), 419-421.
Brion F, Tyler CR, Palazzi X, Laillet B, Porcher JM, Garric J, Flammarion P. (2004) Impacts of 17β-estradiol, including environmentally relevant concentrations, on reproduction after exposure during embryo- larval-, juvenile- and adult-life stages in zebrafish (Danio rerio). Aquat. Toxicol. 68(3), 193–217
Browne, P., Noyes, P. D., Casey, W. M., & Dix, D. J. (2017). Application of adverse outcome pathways to US
EPA’s endocrine disruptor screening program. Environmental health perspectives, 125(9), 096001.
Chakraborty, C., Hsu, C. H., Wen, Z. H., & Lin, C. S. (2009). Aggregation of Zebrafish Embryos for High- throughput Drug Toxicity Screening. Journal of Visualized Experiments, (25), e1422
Chen, Q. L., Yu, T., Zhou, J., Huang, H. W., & Wang, P. (2017). Proteomic and metabolomic responses of different zebrafish tissues to BDE-47 and TBBPA exposure. Science of the Total Environment, 574, 1147-1155.
De Coster S, van Larebeke N. Endocrine-disrupting chemicals: associated disorders and mechanisms of action. J Environ Public Health. (2012) 2012:713696. doi 10.1155/2012/713696
De Esch, C., van der Linde, H., Slieker, R., Willemsen, R., & Hermsen, T. (2012). Zebrafish as a model to study the blood–brain barrier in disease and treatment. Drug discovery today: technologies, 9(4), e227-e233
De Esch, C., van der Linde, H., Slieker, R., Willemsen, R., & Hermsen, T. (2012). Zebrafish as a model to study the blood–brain barrier in disease and treatment. Drug discovery today: technologies, 9(4), e227-e233
EFSA J. (2013). Committee ES. Scientific Opinion on the hazard assessment of endocrine disruptors: scientific criteria for identification of endocrine disruptors and appropriateness of existing test methods for assessing effects mediated by these substances on human health and the environment. 11:3132. doi: 10.2903/j.efsa.2013.3132
Filby, A. L., & Tyler, C. R. (2017). Appropriate 'housekeeping' genes for use in expression profiling the effects of environmental estrogens in fish. BMC Molecular Biology, 18(1), 1-11.
Gluckman P. D., Hanson M. A., Bateson P., Beedle A. S., Law C. M., Bhutta Z. A., Anokhin K. V., Bougnères P., Chandak G. R., Dasgupta P.et al. (2009). Towards a new developmental synthesis: adaptive developmental plasticity and human disease. Lancet 373, 1654–1657.
Filby, A. L., & Tyler, C. R. (2017). Appropriate 'housekeeping' genes for use in expression profiling the effects of environmental estrogens in fish. BMC Molecular Biology, 18(1), 1-11.
Gluckman P. D., Hanson M. A., Bateson P., Beedle A. S., Law C. M., Bhutta Z. A., Anokhin K. V., Bougnères P., Chandak G. R., Dasgupta P.et al. (2009). Towards a new developmental synthesis: adaptive developmental plasticity and human disease. Lancet 373, 1654–1657.
Harding, A. K., Daston, G. P., Boyd, G. R., Lucier, G. W., Safe, S. H., Stewart, J., .... & Van Der Kraak, G. (2006). Endocrine disrupting chemicals research program of the US Environmental Protection Agency: summary of a peer-review report. Environmental Health Perspectives, 114(8), 1276-1282.
Hill AJ, Teraoka H, Heideman W, Peterson RE. (2005) Zebrafish as a model vertebrate for investigating chemical toxicity. Toxicol. Sci. 86(1), 6–19
Howe, K., Clark, M. D., Torroja, C. F., Torrance, J., Berthelot, C., Muffato, M., .... & Teucke, M. (2013). The
zebrafish reference genome sequence and its relationship to the human genome. Nature, 496(7446), 498-503.
Kalueff, A. V., Stewart, A. M., & Gerlai, R. (2014). Zebrafish as an emerging model for studying complex brain disorders. Trends in Pharmacological Sciences, 35(2), 63-75.
Lawson, N. D., & Weinstein, B. M. (2002). In vivo imaging of embryonic vascular development using transgenic zebrafish. Developmental biology, 248(2), 307-318
Lieschke GJ, Currie PD. (2007) Animal models of human disease: zebrafish swim into view. Nat. Rev. Genet. 8(5), 353–367
Lonare S. M., Sole S. S. (2018) Assessment of Ractopamine for Endocrine Dysfunction & Teratogenic Effects in Zebrafish.M.V.Sc. Thesis.Maharshtra Animal & Fishery Sciences University, 27
La Merrill, M. A., Vandenberg, L. N., Smith, M. T., Goodson, W., Browne, P., Patisaul, H. B., .... & Zoeller, R. T.
(2020). Consensus on the key characteristics of endocrine-disrupting chemicals as a basis for hazard identification. Nature Reviews Endocrinology, 16(1), 45-57.
MacRae, C. A., & Peterson, R. T. (2015). Zebrafish as tools for drug discovery. Nature Reviews Drug Discovery, 14(10), 721-731
McGrath P, Li C-Q. (2008) Zebrafish: a predictive model for assessing drug-induced toxicity. Drug Discovery Today 13(9-10), 394–401
Mhanni, A. A., & McGowan, R. A. (2004). Global changes in genomic methylation levels during early development of the zebrafish embryo. Development genes and evolution, 214, 412-417.
Nash, J. P., Kime, D. E., Van der Ven, L. T., Wester, P. W., Brion, F., Maack, G., .... & Tyler, C. R. (2004). Long-
term exposure to environmental concentrations of the pharmaceutical ethynylestradiol causes reproductive failure in fish. Environmental health perspectives, 112(17), 1725-1733.
Patil P. N., Sole S. S. (2021). “Evaluation of Fluralaner for Endocrine Disruption & Embryonic Toxicity in
Zebrafish Model”. M.V.Sc. Thesis. Maharshtra Animal& Fishery Sciences University, 48-50
Patisaul, H. B., Fenton, S. E., & Aylor, D. (2018). Animal models of endocrine disruption. Best Practice & Research Clinical Endocrinology & Metabolism, 32(3), 283-297.
Perkins, E. J., Ankley, G. T., Crofton, K. M., Garcia-Reyero, N., LaLone, C. A., Johnson, M. S., ... & Villeneuve,
D. L. (2013). Current perspectives on the use of alternative species in human health and ecological hazard assessments. Environmental health perspectives, 121(9), 1002-1010.
Peterson, R T., Link, B. A., & Dowling, J. E. (2000). Zebrafish as a model for the study of retinal and optic nerve regeneration: implications for the treatment of glaucoma. Investigative ophthalmology & visual science, 41(10), 2678-2686
Hill AJ, Teraoka H, Heideman W, Peterson RE. (2005) Zebrafish as a model vertebrate for investigating chemical toxicity. Toxicol. Sci. 86(1), 6–19
Howe, K., Clark, M. D., Torroja, C. F., Torrance, J., Berthelot, C., Muffato, M., .... & Teucke, M. (2013). The
zebrafish reference genome sequence and its relationship to the human genome. Nature, 496(7446), 498-503.
Kalueff, A. V., Stewart, A. M., & Gerlai, R. (2014). Zebrafish as an emerging model for studying complex brain disorders. Trends in Pharmacological Sciences, 35(2), 63-75.
Lawson, N. D., & Weinstein, B. M. (2002). In vivo imaging of embryonic vascular development using transgenic zebrafish. Developmental biology, 248(2), 307-318
Lieschke GJ, Currie PD. (2007) Animal models of human disease: zebrafish swim into view. Nat. Rev. Genet. 8(5), 353–367
Lonare S. M., Sole S. S. (2018) Assessment of Ractopamine for Endocrine Dysfunction & Teratogenic Effects in Zebrafish.M.V.Sc. Thesis.Maharshtra Animal & Fishery Sciences University, 27
La Merrill, M. A., Vandenberg, L. N., Smith, M. T., Goodson, W., Browne, P., Patisaul, H. B., .... & Zoeller, R. T.
(2020). Consensus on the key characteristics of endocrine-disrupting chemicals as a basis for hazard identification. Nature Reviews Endocrinology, 16(1), 45-57.
MacRae, C. A., & Peterson, R. T. (2015). Zebrafish as tools for drug discovery. Nature Reviews Drug Discovery, 14(10), 721-731
McGrath P, Li C-Q. (2008) Zebrafish: a predictive model for assessing drug-induced toxicity. Drug Discovery Today 13(9-10), 394–401
Mhanni, A. A., & McGowan, R. A. (2004). Global changes in genomic methylation levels during early development of the zebrafish embryo. Development genes and evolution, 214, 412-417.
Nash, J. P., Kime, D. E., Van der Ven, L. T., Wester, P. W., Brion, F., Maack, G., .... & Tyler, C. R. (2004). Long-
term exposure to environmental concentrations of the pharmaceutical ethynylestradiol causes reproductive failure in fish. Environmental health perspectives, 112(17), 1725-1733.
Patil P. N., Sole S. S. (2021). “Evaluation of Fluralaner for Endocrine Disruption & Embryonic Toxicity in
Zebrafish Model”. M.V.Sc. Thesis. Maharshtra Animal& Fishery Sciences University, 48-50
Patisaul, H. B., Fenton, S. E., & Aylor, D. (2018). Animal models of endocrine disruption. Best Practice & Research Clinical Endocrinology & Metabolism, 32(3), 283-297.
Perkins, E. J., Ankley, G. T., Crofton, K. M., Garcia-Reyero, N., LaLone, C. A., Johnson, M. S., ... & Villeneuve,
D. L. (2013). Current perspectives on the use of alternative species in human health and ecological hazard assessments. Environmental health perspectives, 121(9), 1002-1010.
Peterson, R T., Link, B. A., & Dowling, J. E. (2000). Zebrafish as a model for the study of retinal and optic nerve regeneration: implications for the treatment of glaucoma. Investigative ophthalmology & visual science, 41(10), 2678-2686
Peterson RT, Macrae CA. (2012) Systematic approaches to toxicology in the zebrafish. Annu. Rev. Pharmacol. Toxicol. 52, 433–453
Schiller, V., Wichmann, A., Kriehuber, R., &Muth-Köhne, E. (2013). Influence of water temperature and estrogen exposure on endocrine and reproductive parameters in male zebrafish. Environmental Toxicology and Chemistry, 32(7), 1606-1617.
Schiller, V., Wichmann, A., Kriehuber, R., Muth-Köhne, E., Giesy, J. P., Hecker, M., & Fenske, M. (2013).
Effects of long-term UV filters (4-MBC, OMC, BP-3, BZ-12) on fish, crustaceans, algae and mollusks in a lotic ecosystem. Science of the Total Environment, 463, 45-55. Schug TT, Janesick A, Blumberg B, Heindel JJ. Endocrine disrupting chemicals and disease susceptibility. J Steroid Biochem Mol Biol. (2011) 127:204–15. doi: 10.1016/j.jsbmb.2011.08.007 Segnur, H. (2009). Zebrafish (Danio rerio) as a model organism for investigating endocrine disruption. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 149(2), 187-195
Sipes NS, Padilla S, Knudsen TB. (2011) Zebrafish: as an integrative model for twenty-first century toxicity testing. Birth Defects Res. Part C Embryo Today Rev. 93(3), 256–267 Skakkebaek, N. E., De Meyts, E. R., & Main, K. M. (2001). Testicular dysgenesis syndrome: an increasingly common developmental disorder with environmental aspects. Apmis, 109(S103), S22-S30.
Spence, R., Gerlach, G., Lawrence, C., & Smith, C. (2008). The behaviour and ecology of the zebrafish, Danio rerio. Biological reviews, 83(1), 13-34.
Spitsbergen, J. M., & Kent, M. L. (2003). The state of the art of the zebrafish model for toxicology and toxicologic pathology research—advantages and current limitations. Toxicologic pathology, 31(1_suppl), 62-87.
Tse, W. K., Yeung, B. H., Wan, H. T., & Wong, C. K. (2013). Early embryogenesis in zebrafish is affected by bisphenol A exposure. Biology open, 2(5), 466-471. Virtanen, H. E., Rajpert-De Meyts, E., Main, K. M., Skakkebaek, N. E., & Toppari, J. (2005). Testicular dysgenesis syndrome and the development and occurrence of male reproductive disorders. Toxicology and applied pharmacology, 207(2), 501-505.
Wang, G., Rajpurohit, S. K., Delaspre, F., Walker, S. L., White, D. T., Ceasrine, A., ... & Mumm, J. S. (2015).
First quantitative high-throughput screen in zebrafish identifies novel pathways for increasing
pancreatic β-cell mass. Elife, 4, e08261.
Wang, Q., Liu, S., Jiang, Y., Zhao, Y., Liu, H., Liu, Z., & Wang, C. (2015). Estrogenic endocrine-disrupting effects of organophosphorus flame retardants and related mechanisms in early life stages of zebrafish. Environmental Science & Technology, 49(21), 13370-13379
Wang, Q., Wang, X., Niu, C., & Li, Y. (2016). Endocrine disruption and reproductive impairment in zebrafish by exposure to 8:2 fluorotelomer alcohol. Aquatic Toxicology, 175, 109-118
Westerfield M. (2000) The Zebrafish Book. A Guide for the Laboratory Use of Zebrafish (Danio rerio). University of Oregon Press
Xu, H., Yang, M., Qiu, W., Pan, C., & Wu, M. (2013). The impact of endocrine‐disrupting chemicals on oxidative stress and innate immune response in zebrafish embryos. Environmental Toxicology and Chemistry, 32(8), 1793-1799.
Schiller, V., Wichmann, A., Kriehuber, R., &Muth-Köhne, E. (2013). Influence of water temperature and estrogen exposure on endocrine and reproductive parameters in male zebrafish. Environmental Toxicology and Chemistry, 32(7), 1606-1617.
Schiller, V., Wichmann, A., Kriehuber, R., Muth-Köhne, E., Giesy, J. P., Hecker, M., & Fenske, M. (2013).
Effects of long-term UV filters (4-MBC, OMC, BP-3, BZ-12) on fish, crustaceans, algae and mollusks in a lotic ecosystem. Science of the Total Environment, 463, 45-55. Schug TT, Janesick A, Blumberg B, Heindel JJ. Endocrine disrupting chemicals and disease susceptibility. J Steroid Biochem Mol Biol. (2011) 127:204–15. doi: 10.1016/j.jsbmb.2011.08.007 Segnur, H. (2009). Zebrafish (Danio rerio) as a model organism for investigating endocrine disruption. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 149(2), 187-195
Sipes NS, Padilla S, Knudsen TB. (2011) Zebrafish: as an integrative model for twenty-first century toxicity testing. Birth Defects Res. Part C Embryo Today Rev. 93(3), 256–267 Skakkebaek, N. E., De Meyts, E. R., & Main, K. M. (2001). Testicular dysgenesis syndrome: an increasingly common developmental disorder with environmental aspects. Apmis, 109(S103), S22-S30.
Spence, R., Gerlach, G., Lawrence, C., & Smith, C. (2008). The behaviour and ecology of the zebrafish, Danio rerio. Biological reviews, 83(1), 13-34.
Spitsbergen, J. M., & Kent, M. L. (2003). The state of the art of the zebrafish model for toxicology and toxicologic pathology research—advantages and current limitations. Toxicologic pathology, 31(1_suppl), 62-87.
Tse, W. K., Yeung, B. H., Wan, H. T., & Wong, C. K. (2013). Early embryogenesis in zebrafish is affected by bisphenol A exposure. Biology open, 2(5), 466-471. Virtanen, H. E., Rajpert-De Meyts, E., Main, K. M., Skakkebaek, N. E., & Toppari, J. (2005). Testicular dysgenesis syndrome and the development and occurrence of male reproductive disorders. Toxicology and applied pharmacology, 207(2), 501-505.
Wang, G., Rajpurohit, S. K., Delaspre, F., Walker, S. L., White, D. T., Ceasrine, A., ... & Mumm, J. S. (2015).
First quantitative high-throughput screen in zebrafish identifies novel pathways for increasing
pancreatic β-cell mass. Elife, 4, e08261.
Wang, Q., Liu, S., Jiang, Y., Zhao, Y., Liu, H., Liu, Z., & Wang, C. (2015). Estrogenic endocrine-disrupting effects of organophosphorus flame retardants and related mechanisms in early life stages of zebrafish. Environmental Science & Technology, 49(21), 13370-13379
Wang, Q., Wang, X., Niu, C., & Li, Y. (2016). Endocrine disruption and reproductive impairment in zebrafish by exposure to 8:2 fluorotelomer alcohol. Aquatic Toxicology, 175, 109-118
Westerfield M. (2000) The Zebrafish Book. A Guide for the Laboratory Use of Zebrafish (Danio rerio). University of Oregon Press
Xu, H., Yang, M., Qiu, W., Pan, C., & Wu, M. (2013). The impact of endocrine‐disrupting chemicals on oxidative stress and innate immune response in zebrafish embryos. Environmental Toxicology and Chemistry, 32(8), 1793-1799.
Available Online
30th March, 2024
Google Scholar Link
How to Cite the Article
Bhapkar T, Umap S, Somkuwar AP, Sawarkar A, Deshmukh G and Purankar MM. Swimming Through Science: Unveiling the Secrets of Endocrine Disruptors with Zebrafish Models. Chron Aquat Sci. 2024;1(10):115-129
Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Swimming Through Science: Unveiling the Secrets of Endocrine Disruptors with Zebrafish Models
