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Original Research
In-vitro Anti-microbial Activities of Acacia nilotica Pod extract against Saprolegniasis
Umar, H. M., Saidu, M.,* Aisha, M. M., Attah, J. O., Adam, A. A
Abstract
In-vitro anti-microbial activities of Acacia nilotica pod extract against saprolegniasis was carried out at University of Maiduguri. The aim was to determine the antimicrobial susceptibility of malachite green and Acacia nilotica on saprolegnia isolates. Total samples collected were 91 and sterilized swap sticks was used for the sample collection and were inoculated into prepared media of SDA (Sabouraud Dextrose Agar), the plates was incubated at 30C for 24-48 hours. The extraction of the pods was done using Plant Tissue Homogenization methods. 30g of extract powder was used in Methanol, chloroform and Ethanol. Modified Kirby Bauer disc diffusion technique was employed to determine the antimicrobial resistance pattern of the saprolegniasis isolates and data was analyzed using SPSS. The results shows malachite green as positive control indicated the zone of inhibitions ranging from 27 to 29.00mm, ethanol extract of A. nilotica pod extract at 100% indicated susceptibility of saprolegniasis with the inhibition zones ranging from 20-23mm, Methanol extract at 100% shows intermediate with the inhibitory zones ranging from l1-20mmn and Chloroform extract shows no inhibitory zone at 50, 75 and 100%, the study was concluded that ethanol and methanol of A. nilotica pod extract possess antimicrobial activity against the fish fungus (saprolegnia) which shows antifungal activities but cannot substitute malachite green in this study.
Keywords
In-vitro anti-microbial, Acacia nilotica, saprolegniasis
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  • Published online
  • 30th October, 2023
 
How to Cite the Article
Umar, H. M., Saidu, M., Aisha, M. M., Attah, J. O. and Adam, A. A. 2023. In-vitro Anti-microbial Activities of Acacia nilotica Pod extract against Saprolegniasis. Chronicle of Aquatic Science 1(5): 29-48. 
 
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